Mass spectrometry follows various type of ionization methods. The important method that the chemist use are electron impact (EI) and Fast Atom Bombardment (FAB). These techniques are not utilized much with present day mass spectrometry apart from EI for ecological work utilizing Gas chromatography mass spectrometry. Electrospray ionization (ESI) is the ionization method that has turned into the most well-known ionization procedure. The electrospray is made by creating a high voltage on a stream of fluid at environmental weight, some of the time this is helped by a simultaneous stream of gas.