Chromatography and High Performance Liquid Chromatography (HPLC)
High pressure liquid chromatography is generally used for analysing of biological molecule. HPLC depends on systems of adsorption, partition and ion exchange, depending upon the kind of stationary phase utilized. HPLC includes a solid stationary phase, packed inside a stainless-steel segment, and a liquid mobile phase. HPLC can be used to check the purity and/or determine the particles of many pharmaceutical bioprocessing materials. It can also be used to find enantiomeric composition, using modified mobile phases or chiral stationary phases. It is a very powerful technique then it requires non-polar solvents. concerns this mode is It is used as analytical technique due to its safety and environmental activities.